[2014-04-25 19:53:23] Beginning TopHat run (v2.0.8b) ----------------------------------------------- [2014-04-25 19:53:23] Checking for Bowtie Bowtie version: 2.1.0.0 [2014-04-25 19:53:23] Checking for Samtools Samtools version: 0.1.19.0 [2014-04-25 19:53:23] Checking for Bowtie index files [2014-04-25 19:53:23] Checking for reference FASTA file [2014-04-25 19:53:23] Generating SAM header for /home/rneme/PopTranscriptomes/mapping/index/mm10 format: fastq quality scale: phred33 (default) [2014-04-25 19:53:25] Preparing reads left reads: min. length=60, max. length=101, 28146156 kept reads (8676 discarded) [2014-04-25 20:04:45] Mapping left_kept_reads to genome mm10 with Bowtie2 [2014-04-25 20:40:46] Mapping left_kept_reads_seg1 to genome mm10 with Bowtie2 (1/4) [2014-04-25 20:43:39] Mapping left_kept_reads_seg2 to genome mm10 with Bowtie2 (2/4) [2014-04-25 20:46:50] Mapping left_kept_reads_seg3 to genome mm10 with Bowtie2 (3/4) [2014-04-25 20:50:48] Mapping left_kept_reads_seg4 to genome mm10 with Bowtie2 (4/4) [2014-04-25 20:54:08] Searching for junctions via segment mapping [2014-04-25 20:58:23] Retrieving sequences for splices [2014-04-25 21:00:19] Indexing splices [2014-04-25 21:01:00] Mapping left_kept_reads_seg1 to genome segment_juncs with Bowtie2 (1/4) [2014-04-25 21:02:06] Mapping left_kept_reads_seg2 to genome segment_juncs with Bowtie2 (2/4) [2014-04-25 21:03:24] Mapping left_kept_reads_seg3 to genome segment_juncs with Bowtie2 (3/4) [2014-04-25 21:04:41] Mapping left_kept_reads_seg4 to genome segment_juncs with Bowtie2 (4/4) [2014-04-25 21:05:57] Joining segment hits [2014-04-25 21:09:43] Reporting output tracks ----------------------------------------------- [2014-04-25 21:25:26] Run complete: 01:32:03 elapsed