[2014-04-28 14:45:04] Beginning TopHat run (v2.0.8b) ----------------------------------------------- [2014-04-28 14:45:04] Checking for Bowtie Bowtie version: 2.1.0.0 [2014-04-28 14:45:04] Checking for Samtools Samtools version: 0.1.19.0 [2014-04-28 14:45:04] Checking for Bowtie index files [2014-04-28 14:45:04] Checking for reference FASTA file [2014-04-28 14:45:04] Generating SAM header for /home/rneme/PopTranscriptomes/mapping/index/mm10 format: fastq quality scale: phred33 (default) [2014-04-28 14:45:07] Preparing reads left reads: min. length=60, max. length=101, 20122788 kept reads (11178 discarded) [2014-04-28 14:53:17] Mapping left_kept_reads to genome mm10 with Bowtie2 [2014-04-28 15:19:51] Mapping left_kept_reads_seg1 to genome mm10 with Bowtie2 (1/4) [2014-04-28 15:21:44] Mapping left_kept_reads_seg2 to genome mm10 with Bowtie2 (2/4) [2014-04-28 15:23:53] Mapping left_kept_reads_seg3 to genome mm10 with Bowtie2 (3/4) [2014-04-28 15:26:28] Mapping left_kept_reads_seg4 to genome mm10 with Bowtie2 (4/4) [2014-04-28 15:28:49] Searching for junctions via segment mapping [2014-04-28 15:32:22] Retrieving sequences for splices [2014-04-28 15:34:19] Indexing splices [2014-04-28 15:34:55] Mapping left_kept_reads_seg1 to genome segment_juncs with Bowtie2 (1/4) [2014-04-28 15:35:43] Mapping left_kept_reads_seg2 to genome segment_juncs with Bowtie2 (2/4) [2014-04-28 15:36:40] Mapping left_kept_reads_seg3 to genome segment_juncs with Bowtie2 (3/4) [2014-04-28 15:37:38] Mapping left_kept_reads_seg4 to genome segment_juncs with Bowtie2 (4/4) [2014-04-28 15:38:34] Joining segment hits [2014-04-28 15:41:44] Reporting output tracks ----------------------------------------------- [2014-04-28 15:53:23] Run complete: 01:08:18 elapsed