[2014-04-30 05:14:17] Beginning TopHat run (v2.0.8b) ----------------------------------------------- [2014-04-30 05:14:17] Checking for Bowtie Bowtie version: 2.1.0.0 [2014-04-30 05:14:17] Checking for Samtools Samtools version: 0.1.19.0 [2014-04-30 05:14:17] Checking for Bowtie index files [2014-04-30 05:14:17] Checking for reference FASTA file [2014-04-30 05:14:17] Generating SAM header for /home/rneme/PopTranscriptomes/mapping/index/mm10 format: fastq quality scale: phred33 (default) [2014-04-30 05:14:19] Preparing reads left reads: min. length=60, max. length=101, 19755802 kept reads (10312 discarded) [2014-04-30 05:22:24] Mapping left_kept_reads to genome mm10 with Bowtie2 [2014-04-30 05:47:31] Mapping left_kept_reads_seg1 to genome mm10 with Bowtie2 (1/4) [2014-04-30 05:49:27] Mapping left_kept_reads_seg2 to genome mm10 with Bowtie2 (2/4) [2014-04-30 05:51:36] Mapping left_kept_reads_seg3 to genome mm10 with Bowtie2 (3/4) [2014-04-30 05:54:03] Mapping left_kept_reads_seg4 to genome mm10 with Bowtie2 (4/4) [2014-04-30 05:56:36] Searching for junctions via segment mapping [2014-04-30 06:01:24] Retrieving sequences for splices [2014-04-30 06:03:21] Indexing splices [2014-04-30 06:03:59] Mapping left_kept_reads_seg1 to genome segment_juncs with Bowtie2 (1/4) [2014-04-30 06:04:54] Mapping left_kept_reads_seg2 to genome segment_juncs with Bowtie2 (2/4) [2014-04-30 06:06:00] Mapping left_kept_reads_seg3 to genome segment_juncs with Bowtie2 (3/4) [2014-04-30 06:07:06] Mapping left_kept_reads_seg4 to genome segment_juncs with Bowtie2 (4/4) [2014-04-30 06:08:10] Joining segment hits [2014-04-30 06:11:27] Reporting output tracks ----------------------------------------------- [2014-04-30 06:23:19] Run complete: 01:09:02 elapsed