[2014-04-30 07:35:11] Beginning TopHat run (v2.0.8b) ----------------------------------------------- [2014-04-30 07:35:11] Checking for Bowtie Bowtie version: 2.1.0.0 [2014-04-30 07:35:11] Checking for Samtools Samtools version: 0.1.19.0 [2014-04-30 07:35:11] Checking for Bowtie index files [2014-04-30 07:35:11] Checking for reference FASTA file [2014-04-30 07:35:11] Generating SAM header for /home/rneme/PopTranscriptomes/mapping/index/mm10 format: fastq quality scale: phred33 (default) [2014-04-30 07:35:13] Preparing reads left reads: min. length=60, max. length=101, 18395943 kept reads (10283 discarded) [2014-04-30 07:42:38] Mapping left_kept_reads to genome mm10 with Bowtie2 [2014-04-30 08:05:50] Mapping left_kept_reads_seg1 to genome mm10 with Bowtie2 (1/4) [2014-04-30 08:07:37] Mapping left_kept_reads_seg2 to genome mm10 with Bowtie2 (2/4) [2014-04-30 08:09:39] Mapping left_kept_reads_seg3 to genome mm10 with Bowtie2 (3/4) [2014-04-30 08:12:01] Mapping left_kept_reads_seg4 to genome mm10 with Bowtie2 (4/4) [2014-04-30 08:14:24] Searching for junctions via segment mapping [2014-04-30 08:19:07] Retrieving sequences for splices [2014-04-30 08:21:03] Indexing splices [2014-04-30 08:21:37] Mapping left_kept_reads_seg1 to genome segment_juncs with Bowtie2 (1/4) [2014-04-30 08:22:28] Mapping left_kept_reads_seg2 to genome segment_juncs with Bowtie2 (2/4) [2014-04-30 08:23:30] Mapping left_kept_reads_seg3 to genome segment_juncs with Bowtie2 (3/4) [2014-04-30 08:24:32] Mapping left_kept_reads_seg4 to genome segment_juncs with Bowtie2 (4/4) [2014-04-30 08:25:32] Joining segment hits [2014-04-30 08:28:41] Reporting output tracks ----------------------------------------------- [2014-04-30 08:39:56] Run complete: 01:04:45 elapsed