LB: 1l  

  
    10 g bactotrypton
    10 g NaCl
    5 g yeast extract
    15 g Bactoagar (for plates)

SOB 1l:

    20 g bactotrypton
    5 g yeast extract
    0,5 g NaCl
    10 mM Mg-sulfat
    2,5 mM KCl

SOC

SOB supplemented with 20 mM Glucose (from a 1 M stock solution)


Antibiotics: (store at -20°C)


Ampicillin:
stock (1000x): 100 mg/ml, final concentration: 50-200 µg/ml
Tetracyclin (hydrochlorid):
stock (1000x) : 12 mg/ml in H2O,   final concentration 12,5 µg/ml
Kanamycin:
stock (400x):  10 mg/ml in H2O,   final concentration 25  µg/ml
Chloramphenicol
stock (1000x): 12,5 mg/ml in Ethanol,  final concentration:  12,5 µg/ml

blue/white selection:

LB+Amp plates supplemented with 20µl of IPTG (100mM) and 40 µl of X-Gal (20mg/ml in DMF)