Purification of recombinant PfuSThe enzyme for the highly efficient DNA polymerase mix are purified according to the manual from the Görlich laboratory. Please find here Görlich's manual and here our own protocol with detailed instructions.The purification and quality control was performed by Zhiyi in summer 2015. Here is the documentation of the purification by HiTrap Ni-NTA columns. SDS-PAGE The quality of the enzyme mix was tested with a series of amplifications with a KS-DiaGFP plasmid. Please find the results here. |