Purification of recombinant PfuSThe enzyme for the highly efficient DNA polymerase mix are purified according to the manual from the Görlich laboratory. Please find here the manual with a detailed instruction.The purification and quality control was performed by Zhiyi in summer 2015. Here is the documentation of the purification by HiTrap Ni-NTA columns. SDS-PAGE The quality of the enzyme mix was tested with a series of amplifications with a KS-DiaGFP plasmid. Please find the results here. |