Protocols and materials of the Großhans laboratory

Flies

Bacteria

Yeast

Cell culture

Cloning-DNA-RNA

Proteins

protein purification

Recombinant proteins

Biochemistry

Microscopy

e-mail:
jgrossh@gwdg.de

Cell culture:

General comments on culturing of cells in vitro
Freezing of cells for conservation and recovery
PCR Mycoplasma contamination
Transfection of cells
Electroporation of cells
Stable cell lines
Fixation and immuno-staining of cells
RNAi treatment of cells
Extracts from cultured cells