Protein purification
Columnsavailable for
chromatography at the Äkta prime
Expression
and soluability test of overexpressed proteins
large-scale
expression and purification of recombinant proteins (native conditions)
Purification under denatured conditions
Qiagen
booklet from 2003. This booklet describes and discusses the expression and
purification of His-tagged proteins in detail. Newer versions of
the pQE vectors, such as pQE80 are not included.
covalent coupling of proteins to beads
(BrCN-sepharose)
affinity and IgG purification of
antibodies
Manuals and product descriptions
Desalting by gravity flow/drop columns
(PD-10) or by G-25 Sephadex columns
(HiTrap-G25)
Gel filtration: Superdex,
MW equilibration kits
His-tagged proteins: Ni-NTA Sepharose,
HiTrap-Ni
GST-tagged proteins: GSH-Sepharose,
HiTrap-GSH
Ion exchange: HTrap columns
Antibody purification: general
manual
IgG purification with Protein A:
Protein A HiTrap columns
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