Protocols and materials of the Großhans laboratory

Flies

Bacteria

Yeast

Cell culture

Cloning-DNA-RNA

Proteins

protein purification

Recombinant proteins

Biochemistry

Microscopy

e-mail:
jgrossh@gwdg.de


Biochemistry

Immunprecipitation
Isolation of nuclei from embryonic lysates
GDP-GTP exchange assay (GEF activity)
binding tests with ZZ fusion proteins
purification and labelling of actin
Polymerisation assay in vitro using Pyrene-actin
kinase assay with Cdk immuno precipitates