Protocols and materials of the Großhans laboratory

Flies

Bacteria

Yeast

Cell culture

Cloning-DNA-RNA

Proteins

protein purification

Recombinant proteins

Biochemistry

Microscopy

e-mail:
jgrossh@gwdg.de


MolecularGenetics-DNA-RNA

Shipping of plasmid DNA
minipräp of plasmid DNA
DNA amplification by PCR for cloning with PfuS
RNA isolation from embryos by Trizol
DNA extraction from single adult flies or larvae
large scale preparation of genomic DNA from adult flies
in vitro transcription, mRNA synthesis
dsRNA synthesis for RNAi
synthesis of fluorescent mRNA
site-directed mutagenesis with Pfu and DpnI
colony PCR