Homepage Laboratorium Grosshans

Protocols and materials of the Großhans laboratory

Cloning-DNA-RNA

protein purification

Recombinant proteins

e-mail:
jgrossh@gwdg.de

Flies:

Manual for working with flies (Fliegenbrevier)
preparation of fly food
Fixation of embryos
Immuno-DNA-Actin staining of embryos
RNA in situ hybridisation
BrdU staining. Detection of proliferating cells
X-Gal staining. Detection lacZ reporter genes
time-lapse recordings of embryos
preparation of apple juice plates
Transformation of flies - transgenic stocks
Mosquitos breeding and fixation