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Protocols and materials of the Großhans laboratory

Cloning-DNA-RNA

protein purification

Recombinant proteins

e-mail:
jgrossh@gwdg.de

Yeast and two-hybrid:

Yeast media (For growing and X-Gal assay)
yeast stocks (genotypes, preservation)
Transformation von Hefe (Lithiumacetat Methode)
quick transformation with competent cells
filter assay
Description of the two-hybrid libraries with cDNA from Drosophila ovaries
Isolation of genomic and plasmid DNA from yeast cells
Amplification of JG inserts for sequencing
Plasmide: pJG4-5, pEG202